Culture Medium - VitaVitro Biotech

VitaVitro^® 1-step Culture Medium

VitaVitro^® 1-step Culture Medium is designed for continuous culture from Day 1 to 5, with no dish or medium change required.

FEATURES

No change over during the whole process until the embryo develops to the blastocyst stage to reduce the adverse effects
Works with conventional incubators or time-lapse systems
Ready-to-use with HSA

QUALITY CONTROL

Osmotic pressure：250-290 mOsm/kg
pH: 7.2-7.6
Endotoxin：< 0.25EU/mL
Sterility test：Passed
Mouse embryo assay (1-cell)：≥ 80% development to blastocyst at 96 hours

CLINICAL DATA

1-Step Culture Medium: RCT Study in China

In 2022, a prospective self-matching study was conducted in China, enrolling 128 patients aged 20 to 35 years, with a total of 2,189 oocytes (all participants underwent long-term IVF fertilization). A total of 114 single blastocysts were transplanted using Vitatro 1-step Culture Medium in the experimental group and another sequential culture medium in the control group.

The results showed that in the test group, the fertilization rate was 71.2%, the Day 3 Good Quality Embryo Rate was 53.4%, the Day 5/6 Blastocyst Development Rate was 58.9%, and the Clinical Pregnancy Rate was 68.89%.

*D5/6 Blastocyst Rate= number of blastocysts D5/6 / number of 2PN fertilized oocyte

*hCG Positive Rate= number of hCG positive cases / number of transfer cases

*Implantation Rate =number of gestational sacs observed 35±7 days after transfer / number of transfer blastocysts

EU Clinical Data

In 2021, a prospective, self paired study was carried out in Europe, including 24 subjects aged 30-39 years, with a total of 206 oocytes.

The results shows that in the test group, the fertilization rate was 81%, the Blastocyst Development Rate was 65%, and the Good-quality Blastocyst Rate was 87%.

*Blastocyst Development Rate = (D5/6 Total number of blastocyst) / 2PN Number of fertilized oocytes *100%

*Good-quality Blastocyst Rate = Number of Good-quality blastocysts / Number of formatted blastocysts*100%

REFERENCES

1. Vaita G., Peura T.T., Holm P., Páldi A., Greve T., Trounson A.O., Callesen H. New method of culture of zona-included or zona-free embryos: the Well-of-the-Well (WOW) system. Mol. Reprod. Dev. 2000; 55(3): 256–264. DOI:10.1002/(sici)1098-2795(200003)55:3<256: aid-mrd3>3.0.co;2-7.
2. Vajta G., Kőrösi T., Du Y., Nakata K., leda S., Kuwayama M., Nagy Z.P. The Well-of-the-Well system: an efficient approach to improve embryo development. Reprod Biomed Online. 2008; 17(1): 73-81. DOI: 10.1016/S1472-6483(10)60296-9.
3. Vajta G., Nagy Z.P., Cobo A., Conceicao J., Yovich J. Vitrification in assisted reproduction; myths, mistakes, disbeliefs and confusion. Reprod Biomed Online. 2009; 19 Suppl 3: 1-7. DOI: 10.1016/S1472-6483(10)60278-7.
4. Chen WB, Machaty Z, Marconetto A, Parmegiani L, Vajta G. A Simple and Efficient Solution to Eliminate Evaporation in Mammalian Embryo Cultures, Cell Reprogram.2021 0ct;23(5): 316-318.doi: 10.1089/cel.2021.0061. Epub 2021 Sep 3.

ORDERING INFORMATION

Product Name	Catalog No.	Specifications	Shelf Life
VitaVitro^® 1-step Culture Medium (With HSA)	V004005	5 mL	6 months
	V004010	10 mL
	V004030	30 mL

MATERIAL SAFETY DATA SHEET

VitaVitro^® 1-step Culture Medium MSDS
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